Prostatic Contribution to Normal Serum Acid Phosphatase
نویسندگان
چکیده
منابع مشابه
Prostatic acid phosphatase in serum of patients with prostatic cancer is a specific phosphotyrosine acid phosphatase.
We developed an assay to measure at acid pH the phosphotyrosine phosphatase activity in sera from patients with prostatic cancer. The method used quantifies the inorganic phosphate liberated from phosphotyrosine after incubation with serum, followed by the deproteinization of the reaction mixture. A high acid phosphatase (EC 3.1.3.2) activity towards phosphotyrosine was observed in all sera fro...
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A patient without prostatic carcinoma had a high concentration of prostatic acid phosphatase (PAP; EC 3.1.3.2) in his serum. This PAP was bound to IgG ("macro-PAP"), and IgG autoantibodies against PAP were demonstrated in serum. The patient's IgG prolonged the biological half-life of radiolabeled PAP in rats, suggesting that the formation of IgG-PAP complexes was responsible for decreased PAP c...
متن کاملStabilization and preservation of serum prostatic acid phosphatase activity.
The effects of pH and temperature of storage on the stability of the prostatic fraction of serum acid phosphataseactivity have been studied in order to providea satisfactory method for the preservation of activity in serumsshipped from other hospitals.Addition of citrate tablets of a composition to buffer the serums at pH 6.2 was found to preserve the original activity at 25#{176} for at least ...
متن کاملCounterimmunoelectrophoresis in determination of prostatic acid phosphatase in human serum.
We evaluated counterimmunoelectrophoresis for use in measuring prostatic acid phosphatase in detection of prostatic cancer. After staining for acid phosphatase, we could detect as little as 0.3 ng of purified enzyme standard complexed with antibody by this technique. However, when serum samples were used as antigen, the method was less sensitive (1.5-2.0 ng) because some of the serum proteins m...
متن کاملProstatic Acid Phosphatase: A Potpourri*
In 1938, Gutman et al. (6) described increased levels of acid phosphatase in metastatic carcinoma of the prostate. Since then, when most of us hear the term "acid phosphatase" we immediately think of prostatic cancer, and justifiably so. The link, however, between the test and the diagnosis is not as clear and simple as many of us would like to believe. I would first like to discuss this enzyme...
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ژورنال
عنوان ژورنال: BMJ
سال: 1970
ISSN: 0959-8138,1468-5833
DOI: 10.1136/bmj.4.5733.470